4t1 cl 0007 cell lines  (Procell Inc)

Journal: Heliyon

Article Title: The therapeutic effect and targets of herba Sarcandrae on breast cancer and the construction of a prognostic signature consisting of inflammation-related genes

doi: 10.1016/j.heliyon.2024.e31137

Figure Lengend Snippet: Identification and annotation of DEGs associated with the inflammatory response in breast cancer (BRCA) (A) Volcano plot of DEGs related to the inflammatory response in BRCA based on data from The Cancer Genome Atlas (TCGA). (B) Heatmap of DEGs related to the inflammatory response in BRCA. Blue represents the normal sample; pink represents the tumor sample. (C) Forest plots from univariate Cox regression analysis of TCGA data.

Article Snippet: The mouse BRCA cell line 4T1 (CL-0007, Procell, Wuhan, China) and the human BRCA cell line MDA-MB-231 (human) (CL-0150, Procell) were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Procell) and Leibovitz's L-15 medium (Procell) supplemented with 10 % fetal bovine serum (FBS; Thermo Fisher Scientific, Pittsburgh, PA, USA) and 1 % penicillin‒streptomycin (ScienCell, San Diego, California, USA), respectively, in a humid incubator at 37 °C and 5 % CO 2 .

Techniques:

Journal: Heliyon

Article Title: The therapeutic effect and targets of herba Sarcandrae on breast cancer and the construction of a prognostic signature consisting of inflammation-related genes

doi: 10.1016/j.heliyon.2024.e31137

Figure Lengend Snippet: Construction and validation of a BRCA inflammatory response-related risk scoring model. (A–B) OS of patients in the TCGA-BRCA (A) and GEO-BRCA (B) cohorts. (C) PFS of patients in the TCGA-BRCA cohort. (D–E) Univariate (D) and multivariate (E) analyses were conducted using the 16-INF gene signature and clinical covariates based on overall survival in the TCGA-BRCA cohort. (F) ROC analysis of the specificity and sensitivity of prognosis prediction by the 16-interferon (INF) gene risk score, age, T and N classifications, and tumor stage in the TCGA-BRCA cohort in terms of overall survival. (G) Validation of the prognostic value of the survival-dependent receiver operating characteristic (ROS) curve at 1, 2, and 3 years in the TCGA cohort.

Article Snippet: The mouse BRCA cell line 4T1 (CL-0007, Procell, Wuhan, China) and the human BRCA cell line MDA-MB-231 (human) (CL-0150, Procell) were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Procell) and Leibovitz's L-15 medium (Procell) supplemented with 10 % fetal bovine serum (FBS; Thermo Fisher Scientific, Pittsburgh, PA, USA) and 1 % penicillin‒streptomycin (ScienCell, San Diego, California, USA), respectively, in a humid incubator at 37 °C and 5 % CO 2 .

Techniques: Biomarker Discovery

Journal: Heliyon

Article Title: The therapeutic effect and targets of herba Sarcandrae on breast cancer and the construction of a prognostic signature consisting of inflammation-related genes

doi: 10.1016/j.heliyon.2024.e31137

Figure Lengend Snippet: Relationships between the risk model and clinical features. (A–D) Analysis of the relationships between the 16-INF gene risk score and clinical features such as age, T and N classifications, and tumor stage in the TCGA-BRCA cohort.

Article Snippet: The mouse BRCA cell line 4T1 (CL-0007, Procell, Wuhan, China) and the human BRCA cell line MDA-MB-231 (human) (CL-0150, Procell) were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Procell) and Leibovitz's L-15 medium (Procell) supplemented with 10 % fetal bovine serum (FBS; Thermo Fisher Scientific, Pittsburgh, PA, USA) and 1 % penicillin‒streptomycin (ScienCell, San Diego, California, USA), respectively, in a humid incubator at 37 °C and 5 % CO 2 .

Techniques:

Journal: Heliyon

Article Title: The therapeutic effect and targets of herba Sarcandrae on breast cancer and the construction of a prognostic signature consisting of inflammation-related genes

doi: 10.1016/j.heliyon.2024.e31137

Figure Lengend Snippet: The core gene network of inflammation genes related to BRCA. (A) The core targets of PPIs. (B) The distribution network of degree core targets. (C) The distribution network of stress core targets. (D) The distribution network of betweenness core targets. (E) Molecular docking results of the core protein IRF1 with the active compound rutin.

Article Snippet: The mouse BRCA cell line 4T1 (CL-0007, Procell, Wuhan, China) and the human BRCA cell line MDA-MB-231 (human) (CL-0150, Procell) were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Procell) and Leibovitz's L-15 medium (Procell) supplemented with 10 % fetal bovine serum (FBS; Thermo Fisher Scientific, Pittsburgh, PA, USA) and 1 % penicillin‒streptomycin (ScienCell, San Diego, California, USA), respectively, in a humid incubator at 37 °C and 5 % CO 2 .

Techniques:

Journal: Heliyon

Article Title: The therapeutic effect and targets of herba Sarcandrae on breast cancer and the construction of a prognostic signature consisting of inflammation-related genes

doi: 10.1016/j.heliyon.2024.e31137

Figure Lengend Snippet: Effects of rutin on proliferation, apoptosis, and the IRF1/STAT3/PD-L1 pathway in BRCA cells. (A) CCK-8 analysis of the effects of different concentrations of epimedin C, rutin, or β-sitosterol (0, 10, 50, 100, 500 μM) on BRCA cell viability. (B) Flow cytometry analysis of the effect of rutin (50 μM) on the apoptosis of BRCA cells. (C) Mouse 4T1 and human MDA-231 cells were treated with 0, 10, 100, or 500 μM rutin for 48 h, and the protein level of IRF1 was detected by western blotting. Image was cropped. (D) Co-IP analysis of the interaction between rutin and IRF1. Image was cropped. (E) Western blot analysis of p-STAT3/STAT3/PD-L1 protein levels in MDA-MB-231 cells treated with or without rutin. Image was cropped. (* p < 0.05; ** p < 0.01; *** p < 0.001).

Article Snippet: The mouse BRCA cell line 4T1 (CL-0007, Procell, Wuhan, China) and the human BRCA cell line MDA-MB-231 (human) (CL-0150, Procell) were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Procell) and Leibovitz's L-15 medium (Procell) supplemented with 10 % fetal bovine serum (FBS; Thermo Fisher Scientific, Pittsburgh, PA, USA) and 1 % penicillin‒streptomycin (ScienCell, San Diego, California, USA), respectively, in a humid incubator at 37 °C and 5 % CO 2 .

Techniques: CCK-8 Assay, Flow Cytometry, Western Blot, Co-Immunoprecipitation Assay